Macrophages mediated delivery of chlorin e6 and treatment of lung cancer by photodynamic reprogramming

Photodynamic therapy (PDT) is an emerging anti-tumor strategy. Photosensitizer chlorin e6 (Ce6) can induce photodynamic effect to selectively damage lung cancer cells. In order to further improve its tumor targeting ability, macrophages can be applied as carrier to deliver Ce6 to lung cancer. Tumor associated macrophages (TAM) are important immunocytes in lung cancer immune microenvironment. TAM play crucial role in tumor promotion due to the Immunosuppressive property, reprogramming phenotype of TAM therefore has become a promising strategy. Based on this, in the present study, we suppose that TAM can be used as carrier to deliver Ce6 to lung cancer and be reprogrammed to M1 phenotype by photodynamic action to mediate anti-lung cancer efficacy. The results showed TAM could load with Ce6 and keep viability in the absence of near infrared irradiation (NIR). Moreover, Its viability decreased little within 10 h after NIR. Ce6-loaded TAM could deliver Ce6 to lung cancer cells and retain some drugs in TAM per se. After NIR, phagocytosis of macrophages was enhanced. The expressions of GBP5, iNOS and MHC-II was up-regulated, which indicated TAM were polarized to M1 phenotype. Finally, the study also found the reprogrammed macrophages could inhibit the proliferation and promote the apoptosis of lung cancer cells. These results suggested that macrophages could deliver Ce6 to lung cancer and exhibit anti-lung cancer effect through photodynamic reprogramming. This study provides a novel approach for combining photodynamic action with anti-tumor immunotherapy.

Automated summary

Photodynamic therapy (PDT) using photosensitizer chlorin e6 (Ce6) to selectively target lung cancer cells has shown potential, and in this study, tumor associated macrophages (TAM) were utilized as carriers to deliver Ce6 and reprogrammed to the M1 phenotype through photodynamic action. The reprogrammed macrophages exhibited anti-lung cancer effects by inhibiting proliferation and promoting apoptosis of lung cancer cells, suggesting a novel approach for combining photodynamic therapy with anti-tumor immunotherapy.