期刊
INSECT SCIENCE
卷 29, 期 5, 页码 1329-1345出版社
WILEY
DOI: 10.1111/1744-7917.13001
关键词
Bombyx mori; gene expression; midgut compartmentalization; physiological function; transcriptome sequencing
类别
资金
- National Natural Science Foundation of China [31972619, 31772675]
- Natural Science Foundation of Zhejiang Province [LZ20C170001]
This study analyzed the gene expression in different regions of larval silkworm midgut, revealing regional specialization in digestive enzyme production, transport, metabolism, and hormone regulation. The study also identified the potential role of homeobox transcription factors in transcriptional variations across the midgut.
Insect midgut plays a central role in food digestion and nutrition absorption. Larval silkworm midgut could be divided into 3 distinct regions based on their morphological colors. However, it remains rudimentary of regional gene expression and physiological function in larval silkworm midgut. Through transcriptome sequencing of 3 midgut compartments, a comprehensive analysis of gene expression atlas along the anterior-posterior axis was conducted. Posterior midgut was found transcriptionally divergent from anterior and middle midgut. Differentially expressed gene analysis revealed the regional specialization of digestive enzyme production, transmembrane transport, chitin metabolism, and hormone regulation in different midgut regions. In addition, gene subsets of pan-midgut and region-specific transcription factors (TFs) along the length of midgut were also identified. The results suggested that homeobox TFs might play an essential role in transcriptional variations across the midgut. Altogether, our study provided the first fundamental resource to investigate physiological function and regulation mechanism in larval midgut compartmentalization.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据