Room-Temperature Harvesting Oxidase-Mimicking Enzymes with Exogenous ROS Generation in One Step

Despite the advantages of low cost, high stability, and activities, a majority of nanozymes rely on strict synthesis conditions and precise size/structure control, hindering the stable, bulk, and high-yield production that is necessary for general use. To facilitate the transition of nanozymes from benchtop to real-world applications, we herein present a one-step approach, which only needs mixing of two broad commercialized reagents at room temperature, to harvest gold nanoparticles-bovine serum albumin (BSA) nanocomposite (BSA-Au) with distinct oxidase-like activity and good stability in a broad range of harsh conditions. Density functional theory (DFT) calculations demonstrate the oxidase-like activity of BSA-Au stemming from thermodynamically and kinetically favored facets for O-2 activation. The reactive oxygen species (ROS) generation of BSA-Au contributes to the catalytic activities and further enables water sterilization and antibacterial applications against superbugs. This one-step strategy promises great potential in bulk production of nanozyme for broad application beyond laboratory use.

Automated summary

A one-step approach is presented in this study to produce gold nanoparticle-bovine serum albumin nanocomposite (BSA-Au) with distinct oxidase-like activity and stability, which can be used in water sterilization and antibacterial applications. This strategy overcomes the limitations of strict synthesis conditions and precise size/structure control, allowing for bulk production of nanozymes for broad application.