期刊
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
卷 75, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ifset.2021.102897
关键词
Microalgae; Pulsed electric field; Bioaccessibility; Lipids; Enzymes
资金
- Nestle Research, Lausanne, Switzerland
- ETH Zurich Foundation, Switzerland
- Scientific Center for Optical and Electron Microscopy (ScopeM), ETH Zurich
- Functional Genomics Center Zurich
- James Holzwarth, Nestle Research
The study demonstrated that pulsed electric field treatment can enhance lipid bioaccessibility of Chlorella vulgaris while maintaining cell integrity and oxidative stability, requiring incubation post-treatment. Proteome analysis identified four proteins that may be involved in cell wall lytic activity during incubation after PEF. Future research should focus on further understanding the mechanism behind incubation after PEF and the potential effect played by endogenous cell wall-degrading enzymes.
There is growing demand for gentle technologies to improve the lipid bioaccessibility (BA) of Chlorella vulgaris biomass while preserving cell integrity and therefore oxidative stability. Pulsed electric field treatment (PEF, 5 mu s at 20 kV cm(-1), 31.8 kJ kg(sus)(-1)) led to an enhancement in lipid BA from 4-7.8% (untreated) to 18.7-20.9%. To reach such a level of BA, incubation in buffer after the treatment (12 h at 25/37 degrees C, 48 h at 4 degrees C) was required. As hypothesized, PEF preserved cell integrity, as shown by particle size and scanning electron microscopy analyses, as well as oxidative stability of the biomass over 3 months at 40 degrees C. Proteome analysis identified four proteins that may be involved in cell wall lytic activity during incubation after PEF. Future work should focus on further understanding the mechanism behind incubation after PEF and studying the potential effect played by endogenous cell wall-degrading enzymes.
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