期刊
INDUSTRIAL CROPS AND PRODUCTS
卷 176, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.indcrop.2021.114422
关键词
Cytoplasmic male sterility (CMS); Cotton; Degradome; Micro-RNA
资金
- General Program of the National Natural Science Foundation of China [31871679]
- National Key Research and Development Program of China [2016YFD0101414]
- Central Public-interest Scientific Institution Basal Research Fund of China [1610162021015]
This study investigated the roles of miRNA-target gene pairs in the occurrence of cytoplasmic male sterility (CMS) in cotton. By analyzing small RNA, transcriptome, and degradome sequencing data, the researchers identified novel miRNA and target gene pairs associated with male sterility. The findings provide new insights into the genetic control of CMS in cotton and have implications for breeding new sterile lines.
Cytoplasmic male sterility (CMS) is an important phenotypic trait for heterosis utilization in cotton (Gosspium hirsutum L.). Micro-RNAs (miRNAs) play crucial roles in the pollen development of plants. To elucidate the roles of miRNA-target gene pairs in the CMS cotton line ganmian15A and its maintainer line ganmian15B, an integrated small RNA (sRNA), transcriptome, and degradome sequencing analysis of these lines at the pollen abortion stage was performed in the present research. A total of 339 known miRNAs and 961 conserved miRNAs were identified through high-throughput sequencing, among which 124 were differentially expressed between ganmian15A and ganmian15B. By comprehensive analysis of miRNA, transcriptome, and degradome sequencing, we identified 11 pairs of miRNAs/target genes at the pollen abortion stage. Among them, 9 miRNAs (4 novel and 5 known) were found for the first time to be associated with the occurrence of male sterility in cotton. The target genes of miRna-38 and miRna-23 were linked to cell wall development at the pollen stage. They may play an imperative role in CMS cotton by delaying the degradation of tapetal cells. Our findings revealed new insights into the genetic control of CMS occurrence, which will support the breeding of new sterile lines of cotton in the future.
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