A calcium channel blocker, manoalide exerts an anti-allergic inflammatory effect through attenuating NF-kappa B activity

Background Many people are troubled by allergic inflammation including ocular allergic diseases, anaphylaxis, allergic rhinitis, atopic dermatitis, and eczema. Consequently, finding medications for use in allergic inflammation therapy is crucial in human health. Manoalide, a marine natural product isolated as an anti-bacterial metabolite from Luffariella variabilis, is a calcium channel blocker. However, its latent ability as an anti-allergic inflammatory agent has not yet been reported. Our research aimed to elucidate whether manoalide exerts an anti-allergic inflammatory effect in the human mast cell line, HMC-1. Methods Herein, we investigated the immunoregulatory effects and molecular mechanisms of manoalide in HMC-1 cells. Results Manoalide significantly alleviated secretion of the inflammatory cytokines interleukin (IL)-1 beta, thymic stromal lymphopoietin, tumor necrosis factor-alpha, IL-6, and IL-8 via blockage of caspase-1 without cytotoxicity in activated HMC-1 cells. Activation of nuclear factor-kappa B increased by mast cell stimulation was attenuated by treatment with manoalide. In addition, we demonstrated that manoalide treatment remarkably attenuated the activation of mitogen-activated protein kinases in activated-HMC-1 cells. Conclusions Taken together, our findings indicate manoalide has an anti-allergic inflammatory role, and we propose that manoalide might have potential as a novel anti-allergic inflammatory agent.

Automated summary

Manoalide significantly reduced the secretion of inflammatory cytokines and inhibited the activation of nuclear factor-kappa B in activated HMC-1 cells. Manoalide also attenuated the activation of mitogen-activated protein kinases in these cells, suggesting its potential as a novel anti-allergic inflammatory agent.