期刊
IMMUNOLOGICAL REVIEWS
卷 306, 期 1, 页码 234-243出版社
WILEY
DOI: 10.1111/imr.13039
关键词
B cell; fate mapping; germinal center; imaging window; intravital imaging; multiphoton laser scanning microscopy; photoactivation
类别
资金
- John D. and Catherine T. MacArthur Foundation
- Pew Charitable Trusts
- Burroughs Wellcome Fund
- Damon Runyon Cancer Research Foundation [DRG2353-19]
Germinal centers serve as the site for antibody affinity maturation, and multiphoton laser scanning microscopy has allowed insight into the dynamic biological processes within GCs. This technical advancement has greatly contributed to our understanding of GC biology.
Germinal centers (GCs) are the site of antibody affinity maturation, a fundamental immunological process that increases the potency of antibodies and thereby their ability to protect against infection. GC biology is highly dynamic in both time and space, making it ideally suited for intravital imaging. Using multiphoton laser scanning microscopy (MPLSM), the field has gained insight into the molecular, cellular, and structural changes and movements that coordinate affinity maturation in real time in their native environment. On the other hand, several limitations of MPLSM have had to be overcome to allow full appreciation of GC events taking place across different timescales. Here, we review the technical advances afforded by intravital imaging and their contributions to our understanding of GC biology.
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