期刊
IMMUNOGENETICS
卷 74, 期 2, 页码 245-259出版社
SPRINGER
DOI: 10.1007/s00251-021-01234-5
关键词
Major histocompatibility complex; MHC; Dog leukocyte antigen; DLA; Recombination; Gene conversion; Haplotypes
资金
- Ministry of Education, Culture, Sports, Science, and Technology (MEXT) Japan [S1491007]
By analyzing the DNA sequences of the DLA class I genomic region in dogs, this study discovered that DLA-88L is a hybrid gene formed by interlocus and/or intralocus gene conversion between DLA-88 and DLA-12. It was also found that at least one DLA-12 allele is generated through interlocus gene conversion. These findings suggest that intralocus gene conversion plays an important role in expanding DLA allelic variations.
The dog leukocyte antigen (DLA) class I genomic region is located on chromosome 12, and the class I genomic region is composed of at least two distinct haplotypic gene structures, DLA-88-DLA-12 and DLA-88-DLA-88L. However, detailed information of the genomic differences among DLA-88, DLA-12, and DLA-88L are still lacking at the full-length gene level, and therefore, DLA allelic sequences classified for each of these loci are limited in number so far. In this study, we determined the DNA sequence of a 95-kb DLA class I genomic region including DLA-88, DLA-12/88L, and DLA-64 with three DLA homozygous dogs and of 37 full-length allelic gene sequences for DLA-88 and DLA-12/88L loci in 26 DLA class I homozygous dogs. Nucleotide diversity profiles of the 95-kb regions and sequence identity scores of the allelic sequences suggested that DLA-88L is a hybrid gene generated by interlocus and/or intralocus gene conversion between DLA-88 and DLA-12. The putative minimum conversion tract was estimated to be at least an 850-bp segment in length located from the 5 ' flanking untranslated region to the end of intron 2. In addition, at least one DLA-12 allele (DLA-12*004:01) was newly generated by interlocus gene conversion. In conclusion, the analysis for the occurrence of gene conversion within the dog DLA class I region revealed intralocus gene conversion tracts in 17 of 27 DLA-88 alleles and two of 10 DLA-12 alleles, suggesting that intralocus gene conversion has played an important role in expanding DLA allelic variations.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据