期刊
IMMUNITY
卷 54, 期 12, 页码 2795-+出版社
CELL PRESS
DOI: 10.1016/j.immuni.2021.10.003
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资金
- NIH [DP1 DK1109668, R01 HL145397, R01 HL142905, R37 AI0499653, P30 DK097948]
- Rainin Foundation
- Digestive Diseases Research Core Center of Washington University - NIH [P30 DK052574]
- [T32 DK077653]
- [FA-2020-01-IBD-1]
The formation of tertiary lymphoid organs (TLOs) in the mesentery is a feature of Crohn's disease, blocking cellular and molecular outflow from the gut and causing lymph leakage and backflow. TNF neutralization at early stages can restore lymph transport, but developed chronic TLOs resist regression and restoration of flow after TNF neutralization. TNF stimulation of lymphatic endothelial cells disrupts immune cell transport and valve-associated gene induction, contributing to Crohn's disease pathology.
Lymphangitis and the formation of tertiary lymphoid organs (TLOs) in the mesentery are features of Crohn's disease. Here, we examined the genesis of these TLOs and their impact on disease progression. Whole-mount and intravital imaging of the ileum and ileum-draining collecting lymphatic vessels (CLVs) draining to mesenteric lymph nodes from TNF Delta ARE mice, a model of ileitis, revealed TLO formation at valves of CLVs. TLOs obstructed cellular and molecular outflow from the gut and were sites of lymph leakage and backflow. Tumor necrosis factor (TNF) neutralization begun at early stages of TLO formation restored lymph transport. However, robustly developed, chronic TLOs resisted regression and restoration of flow after TNF neutralization. TNF stimulation of cultured lymphatic endothelial cells reprogrammed responses to oscillatory shear stress, preventing the induction of valve-associated genes. Disrupted transport of immune cells, driven by loss of valve integrity and TLO formation, may contribute to the pathology of Crohn's disease.
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