期刊
HISTOPATHOLOGY
卷 80, 期 3, 页码 515-528出版社
WILEY
DOI: 10.1111/his.14577
关键词
breast lesions; diagnosis; grade; nuclear shape; nuclear size
资金
- Japanese Breast Cancer Society
- 50million investment from the Data to Early Diagnosis and Precision Medicine strand of the government's Industrial Strategy Challenge Fund
This study used ImageJ to assess nuclear morphological features in various breast lesions, finding significant differences in nuclear morphometrics between low-grade IBC-NST and tubular carcinoma, low-grade DCIS and UDH, and CCC and FEA. Additionally, no differences in nuclear features were identified between grade-matched DCIS and IBC-NST.
Aims Although evaluation of nuclear morphology is important for the diagnosis and categorisation of breast lesions, the criteria used to assess nuclear atypia rely upon the subjective evaluation of several features that may result in inter- and intraobserver variation. This study aims to refine the definitions of cytonuclear features in various breast lesions. Methods and results ImageJ was used to assess the nuclear morphological features including nuclear diameter, axis length, perimeter, area, circularity and roundness in 160 breast lesions comprising ductal carcinoma in situ (DCIS), invasive breast carcinoma of no special type (IBC-NST), tubular carcinoma, usual ductal hyperplasia (UDH), columnar cell change (CCC) and flat epithelial atypia (FEA). Reference cells included normal epithelial cells, red blood cells (RBCs) and lymphocytes. Reference cells showed size differences not only between normal epithelial cells and RBCs but also between RBCs in varied-sized blood vessels. Nottingham grade nuclear pleomorphism scores 1 and 3 cut-offs in IBC-NST, compared to normal epithelial cells, were < x1.2 and > x1.4 that of mean maximum Feret's diameter and < x1.6 and > x2.4 that of mean nuclear area, respectively. Nuclear morphometrics were significantly different in low-grade IBC-NST versus tubular carcinoma, low-grade DCIS versus UDH and CCC versus FEA. No differences in the nuclear features between grade-matched DCIS and IBC-NST were identified. Conclusion This study provides a guide for the assessment of nuclear atypia in breast lesions, refines the comparison with reference cells and highlights the potential diagnostic value of image analysis tools in the era of digital pathology.
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