Prestrain in the rabbit eardrum measured by digital image correlation and micro-incisions

Prestrain in the absence of external loads can have an important effect on the vibrational behavior of mechanical systems such as the middle ear. Studies that measure tympanic membrane (TM) prestrain are scarce, however, and provide no conclusive answer on the existence and nature of the prestrain. In this study, prestrain is measured in the TM of cadaveric rabbit ears by stereo digital image correlation. To release the prestrain, straight incisions of 0.33 mm are made on different locations in the TM with a direction parallel to either the radial or circular fibers in the membrane. The effect of sample dehydration during different stages in the experimental procedure is assessed and eliminated by rehydrating the samples directly before each measurement. The measurements demonstrate average prestrain values around the incisions between 3.52 +/- 2.34% and 13.62 +/- 7.92% for the different locations, with a noise floor of 0.07%. No clear differences were found between the prestrain values obtained for radial and circular incisions. Observed local variations in TM prestrain could not be clearly related to specific locations on the TM. The results suggest that TM prestrain may need to be considered in future studies of middle-ear function if the findings can be confirmed in human ears. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

Prestrain in the tympanic membrane (TM) of cadaveric rabbit ears was measured using stereo digital image correlation, with incisions made to release the prestrain. Average prestrain values around the incisions ranged from 3.52% to 13.62%, showing no clear differences between radial and circular incisions.