Isolation from a fish kill and transcriptomic characterization of Gyrodinium jinhaense off Long Island Sound

First found in Korean coastal water, the dinoflagellate Gyrodinium jinhaense is a recently established species with unclear global distribution and unexplored genomic characteristics. From a laboratory fish mortality event off Long Island Sound, USA, we isolated a dinoflagellate, and by microscopic and molecular (18S rRNA gene; >99% identical) analyses found that it resembles G. jinhaense, hence named G. jinhaense strain AP17. Towards developing a genetic database for this dinoflagellate, a transcriptome of this species was sequenced using RNA-seq, producing 6 Gbp of data that was assembled into over 70,000 unigenes. The assembled transcriptome GC content was approximately 56% and the total Benchmarking Universal Single-Copy Orthologs for Eukaryota and Alveolata databases were approximately 50% and 57%, respectfully. Genes involved in grazing, energy generation, genome architecture, and protein synthesis, processing, and degradation were highly represented in the transcriptome. Moreover, fragments of polyketide synthase and saxitoxin genes were found but saxitoxins were not detected in high performance liquid chromatography measurements. With the first reported transcriptome for the Gyrodinium genus, this study will serve as a baseline for future Gyrodinium genomics and toxicological studies.

Automated summary

The dinoflagellate Gyrodinium jinhaense, originally found in Korean coastal water, has unclear global distribution and genomic characteristics. A transcriptome of a closely related species, Gyrodinium jinhaense strain AP17, was sequenced and analyzed, providing valuable insights into genes related to grazing, energy generation, genome architecture, and protein synthesis. This study represents a foundational step for future genomics and toxicological research on Gyrodinium species.