Pangenome inventory of Burkholderia sensu lato, Burkholderia sensu stricto, and the Burkholderia cepacia complex reveals the uniqueness of Burkholderia catarinensis

Here the pangenome analysis of Burkholderia sensu lato (s.l.) was performed for the first time, together with an updated analysis of the pangenome of Burkholderia sensu stricto, and Burkholderia cepacia complex (Bcc) focusing on the Bcc B. catarinensis specific features of its re-sequenced genome. The pangenome of Burkholderia s.l., Burkholderia s.s., and of the Bcc was open, composed of more than 96% of accessory genes, and more than 62% of unknown genes. Functional annotations showed that secondary metabolism genes belonged to the variable portion of genomes, which might explain their production of several compounds with varied bioactivities. Taken together, this work showed the great variability and uniqueness of these genomes and revealed an underexplored unknown potential in poorly characterized genes. Regarding B. catarinensis 89(T), its genome harbors genes related to hydmlases production and plant growth promotion. This draft genome will be valuable for further investigation of its biotechnological potentials.

Automated summary

This study performed pangenome analysis on Burkholderia sensu lato for the first time, and focused on the specific features of B. catarinensis in the pangenome of Burkholderia sensu stricto and Burkholderia cepacia complex (Bcc). The results showed great variability and unknown potential in these genomes. The genome of B. catarinensis contains genes related to hydmlase production and plant growth promotion.