期刊
GENES & DEVELOPMENT
卷 36, 期 3-4, 页码 180-194出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.349190.121
关键词
meiosis; N-6-methyladenosine; RNA-binding proteins; RNA helicase; spermatogenesis; Ythdc2
资金
- National Cancer Institute Cancer Center support grant [P30 CA08748]
- Human Genetics Institute of New Jersey laboratory startup funds
- Howard Hughes Medical Institute
- Cell Starr Cancer Consortium grant [I14-0031]
- National Institutes of Health [R35 GM118080, R35 NS097404, P50 HD104454]
In this study, the researchers investigated how the N-6-methyladenosine (m(6)A) reader and RNA helicase YTHDC2 regulate gene expression during meiosis. They found that YTHDC2 can bind transcripts independent of m(6)A status and regulate gene expression through distinct mechanisms during multiple stages of meiosis. Mutation of the m(6)A-binding pocket of YTHDC2 had no effect on gametogenesis, suggesting that YTHDC2's function is m(6)A-independent. However, mutation in the ATPase motif of YTHDC2 blocked meiotic prophase I progression, causing sterility.
In this study, Saito et al. sought to understand how the N-6-methyladenosine (m(6)A) reader and RNA helicase YTHDC2 switches cells from mitotic to meiotic gene expression programs. Their findings provide insight into YTHDC2's mechanism, and they propose a model in which YTHDC2 binds transcripts independent of m(6)A status and regulates gene expression during multiple stages of meiosis by distinct mechanisms. Mechanisms regulating meiotic progression in mammals are poorly understood. The N-6-methyladenosine (m(6)A) reader and 3 ' -> 5 ' RNA helicase YTHDC2 switches cells from mitotic to meiotic gene expression programs and is essential for meiotic entry, but how this critical cell fate change is accomplished is unknown. Here, we provide insight into its mechanism and implicate YTHDC2 in having a broad role in gene regulation during multiple meiotic stages. Unexpectedly, mutation of the m(6)A-binding pocket of YTHDC2 had no detectable effect on gametogenesis and mouse fertility, suggesting that YTHDC2 function is m(6)A-independent. Supporting this conclusion, CLIP data defined YTHDC2-binding sites on mRNA as U-rich and UG-rich motif-containing regions within 3 ' UTRs and coding sequences, distinct from the sites that contain m(6)A during spermatogenesis. Complete loss of YTHDC2 during meiotic entry did not substantially alter translation of its mRNA binding targets in whole-testis ribosome profiling assays but did modestly affect their steady-state levels. Mutation of the ATPase motif in the helicase domain of YTHDC2 did not affect meiotic entry, but it blocked meiotic prophase I progression, causing sterility. Our findings inform a model in which YTHDC2 binds transcripts independent of m(6)A status and regulates gene expression during multiple stages of meiosis by distinct mechanisms.
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