期刊
GENE
卷 806, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.gene.2021.145928
关键词
CYP19; Promoter analysis; Ovarian follicles; Goose
资金
- National Natural Science Foundation of China [31972567, 31802064]
- Key Technology Support Program of Sichuan Province [2021YFYZ0014]
- China Agriculture Research System of MOF and MARA [CARS-42-4]
The study identified the full-length coding sequence and promoter sequence of the goose CYP19 gene, investigated its expression and distribution profiles in different sized follicles, and analyzed its transcriptional regulatory mechanism. The results showed that the transcription activity of the CYP19 gene is activated by transcriptional factors in its proximal region of the promoter, promoting estrogen synthesis.
Cytochrome P450 Family 19 (CYP19) is a crucial enzyme to catalyze the conversion of androgens to estrogens. However, the regulatory mechanism of goose CYP19 gene remains poorly understood. The present study attempted to obtain the full-length coding sequence (CDS) and 5'-flanking sequence of CYP19 gene, to investigate its expression and distribution profiles in different sized follicles, and to analyze the transcriptional regulatory mechanism of CYP19 gene in goose. Results showed that its CDS consisted of 1512 nucleotides and the encoded amino acid sequence contained a classical P450 structural domain. Homology analysis showed that there were high homologies of nucleotide and amino acid sequences between goose and other avian species. Its promoter sequence spanned from -1925 bp to the transcription start site (ATG) and several transcriptional factors were predicted in this region. Further analysis from luciferase assay showed that the luciferase activity was the highest spanning from -118 to -1 bp by constructing deletion promoter reporter vector. In addition, result from quantitative real-time polymerase chain reaction indicated that the mRNA level of CYP19 gene were highly expressed in theca layer of the fifth largest follicle, and the cellular location was in the theca externa cells by immunohistochemistry. Taken together, it could be concluded that the transcription activity of CYP19 gene was activated by transcriptional factors in its proximal region of promoter to promote the synthesis of estrogens, regulating the selection of pre-hierarchical into hierarchical follicle in goose.
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