期刊
GENE
卷 812, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.gene.2021.146112
关键词
Testosterone; Gdnf; Gfr alpha 1; c-RET; Spermatogonial stem cell; Self-renewal
资金
- Urmia University [1624]
- departments of Basic Sciences and Microbiology, Faculty of Veterinary Medicine, Urmia University
The study shows that testosterone stimulates the production of Gdnf in Sertoli cells, while Gdnf amplifies its receptor expression to affect the self-renewal of spermatogonial stem cells and spermatogenesis. Both testosterone and Gdnf can up-regulate the expression of Gdnf receptors at different concentrations.
The Glial cell-derived neurotrophic factor (Gdnf) and testosterone induce the spermatogonial stem cells (SSCs) self-renewal and spermatogenesis, respectively. In present study the stimulating role of testosterone on Sertoli cells to produce Gdnf, and the possible effect of Gdnf on Gfr alpha 1 and c-RET expressions were investigated. The TM4 cells (line Sertoli cells) were co-cultured with [0.1, 0.2 and 0.4 (ng/ml)] of exogenous and TM3 (line Leydig cells)-produced testosterones, and consequently the TM4-produced Gdnf concentration was evaluated. Next, the SSCs were co-cultured with the TM-4 derived media (endogenous Gdnf) and exogenous Gdnf [0.1, 0.2, and 0.4 ng/ml)]. The 0.1 and 0.2 ng/ml endogenous and 3 concentrations of exogenous testosterone up-regulated the Gdnf expression versus non-treated Sertoli cells. The TM4-produced and exogenous Gdnfs, in all concentrations, up-regulated the receptors expression. In conclusion, the testosterone, solely, stimulates the Gdnf synthesis and the Gdnf, individually, amplifies its receptor's expression at mRNA and protein levels.
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