4.6 Article

DNA N6-Adenine methylation in HBV-related hepatocellular carcinoma

期刊

GENE
卷 822, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.gene.2022.146353

关键词

6mA; Hepatocellular carcinoma; Nanopore sequencing

资金

  1. Transformation project of scientific and technological achievements in Qinghai Province [2020-SF-162]
  2. Na-tional Natural Science Foundation of China [81902618, 81870048]
  3. Special Research Fund for Central Universities, Peking Union Medical College [3332020023]
  4. Beijing Hospital Nova Project [BJ-2020-083]
  5. Beijing Hospital Project [BJ-2019-153]
  6. Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences [2019PT320027]
  7. Beijing Hope Run Special Fund of Cancer Foundation of China [LC2019A04]
  8. Beijing Youth Talent Project [2018000032600G394]

向作者/读者索取更多资源

DNA methylation on N6-adenine (6mA) has been identified as a potential epigenetic marker in prokaryotes and eukaryotes. This study used Nanopore sequencing to globally profile 6mA sites in the genome of hepatocellular carcinoma, revealing widespread distribution throughout the human genome. The 6mA sites were found to be associated with metabolic processes and gene transcription, and may serve as potential biomarkers for cancer diagnosis and treatment.
DNA methylation on N6-adenine (6mA) has recently been found to be a potential epigenetic marker in prokaryotes and eukaryotes. However, its distribution patterns and potential functions in human tumorigenesis remain largely unknown. Here, we reported global profiling of 6mA sites in the genome of hepatocellular carcinoma at single-nucleotide resolution using Nanopore sequencing. 6mA was widely distributed throughout the human genome. The 6mA sites were related to the porphyrin and chlorophyll metabolism in autosomes and were related to oxidative phosphorylation and ATP metabolism in mitochondria. AGG was the most significant motif associated with 6mA modification and the prevalent motifs in tumors were mainly distributed in mitochondria. The density of 6mA was related to the activation of gene transcription and 6mA density in repetitive sequences decreased in hepatocellular carcinoma. DNA 6mA methylation modification may also be a potential biomarker for cancer diagnosis and treatment.

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