期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 179, 期 -, 页码 164-169出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2021.12.310
关键词
Ultra-performance liquid; chromatography -tandem mass spectrometry; (UPLC-MS; MS); Amniotic fluid; Reactive oxygen species (ROS); Oxidative stress; Protein oxidation; DNA oxidation; Method validation
资金
- Instituto de Salud Carlos III [CP16/00034, CD19/00037, PI17/00127, PI15/00263, PI19/00118, PI20/00964]
- RETICS (Spain) [PN 2018-2021]
- ISCIII-Sub-Directorate General for Research Assessment and Promotion
- European Regional Development Fund (FEDER) [RD16/0022]
This study presents an analytical method for quantifying biomarkers of oxidative stress-derived damage to proteins and DNA in amniotic fluid samples. The method achieves high accuracy, precision, and sensitivity, making it suitable for research on oxidative stress-related complications during pregnancy.
Oxidative stress in the fetal period is associated with preterm birth as well as short and long-term adverse clinical outcomes. Here, an Ultra-Performance Liquid Chromatography-tandem Mass Spectrometry (UPLC-MS/MS) method for the simultaneous quantification of biomarkers of oxidative stress-derived damage to proteins and DNA in amniotic fluid (AF) samples is presented. Appropriate accuracy and precision levels, as well as sensitivity with limits of detection in the low nanomolar (<2 nM) range were achieved. The analytical method was applied to a set of AF samples and reference ranges of the biomarker panel are presented. Median concentrations of biomarkers of protein oxidation (ortho-, 3-chloro-, and 3-nitrotyrosine) and their precursors (para-tyrosine and phenylalanine) ranged between 0.6 and 3 nM and 23 and 30 mu M, respectively, while levels of a biomarker of DNA-oxidation (8-hydroxydeoxyguanosine, 8OHdG) and its precursor (2 '-deoxyguanosine) were found to be 0.18 and 3 nM, respectively. Detection frequencies of all metabolites were 100% with exception of 3-chlorotyrosine (3Cl-Tyr) and 8OHdG, that were only detected in 8% of samples. The developed method may be applied in research studies focusing on oxidative stress-related complications during pregnancy.
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