Effect of oxygen availability and pH on adaptive acid tolerance response of immobilized Listeria monocytogenes in structured growth media

The aim of the present study was to evaluate the effect of oxygen availability (aerobic, hypoxic and anoxic conditions) and sub-optimal pH (6.2 and 5.5) in a structured medium (10% w/V gelatin) on the growth of two immobilized L. monocytogenes strains (C5, 6179) at 10 degrees C and their subsequent acid resistance (pH 2.0, e.g., gastric acidity). Anaerobic conditions resulted in lower bacterial population (P < 0.05) (7.8-8.2 log CFU/mL) at the end of storage than aerobic and hypoxic environment (8.5-9.0 log CFU/mL), a phenomenon that was intensified at lower pH (5.5), where no significant growth was observed for anaembically grown cultures. Prolonged habituation of L. monocytogenes (15 days) at both pH increased its acid tolerance resulting in max. 10 times higher t(4D) (appx. 60 min). The combined effect though of oxygen availability and suboptimal pH on L. monocytogenes acid resistance was found to vary with the strain. Anoxically grown cultures at pH 5.5 exhibited the lowest tolerance towards lethal acid stress, with countable survivors occurring only until 20 min of exposure at pH 2.0. Elucidating the role of oxygen limiting conditions, often encountered in structured foods, on acid resistance of L. monocytogenes, would assist in assessing the capacity of L. monocytogenes originated from different food-related niches to withstand gastric acidity and possibly initiate infection.

Automated summary

The study evaluated the impact of oxygen availability and sub-optimal pH on the growth and acid resistance of immobilized L. monocytogenes. Anaerobic conditions resulted in lower bacterial population, especially at lower pH, while prolonged habituation increased acid tolerance. The combined effect of oxygen availability and suboptimal pH on acid resistance varied between strains, with anoxic cultures at pH 5.5 exhibiting the lowest tolerance towards lethal acid stress.