期刊
FOOD CONTROL
卷 130, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2021.108357
关键词
Salmonella; Voltammetric immunosensor; CoFe-MOFs-graphene nanocomposite; Gold nanoparticles
资金
- National Natural Science Foundation of China [31860467]
The novel electrochemical immunosensor based on CoFe-MOFs-graphene nanocomposite offers an effective detection method for Salmonella in milk, with a wide linear range and low detection limit. The sensor demonstrates good stability, selectivity, and reproducibility.
The presence of Salmonella in food has been a serious threat to human health all over the world. Herein, a novel electrochemical immunosensor based on CoFe-MOFs-graphene nanocomposite was constructed to detect Salmonella in milk. The synergy of the large specific surface area and high porosity of CoFe-MOFs and the good electrical conductivity of graphene materials greatly enhanced the current signal of the nanocomposite. Gold nanoparticles were modified on CoFe-MOFs-graphene hybrids by a simple electrodeposition method. Anti-Salmonella antibody was immobilized onto the surface of the constructed electrode through the formation of a stable Au-NH2 bond. Scanning electron microscopy was employed to reveal the structure of the CoFe-MOFs-graphene nanocomposite, and the elements of the nanocomposite were observed by energy-dispersive X-ray spectroscopy. In addition, Fourier transform infrared (FT-IR) and X-ray powder diffraction (XRD) were used to analyze the structure of related materials. Under optimal experimental parameters, the constructed immunosensor detected a wide linear range of Salmonella from 2.4 x 102 CFU mL-1 to 2.4 x 108 CFU mL-1 with a detection limit of 1.2 x 102 CFU mL-1 (S/N = 3). The immunosensor showed good stability, selectivity, and reproducibility. The designed immunosensor provides a new strategy for the rapid detection of foodborne pathogens.
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