期刊
FOOD CHEMISTRY
卷 370, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.131365
关键词
Dual-channel sensing platform; Zearalenone; Gold nanoparticles aggregates; Charge neutralization; SYBR Green I
资金
- National Key Research and Development Program of China [2019YFC1605502]
- Program of State Key Laboratory of Food Science and Technology, Nanchang University [SKLF-ZZA-201912]
- State Key Laboratory of Food Science and Technology of Nanchang University [SKLF-ZZB-201913]
- National Natural Science Foundation of China [82003467]
- Key Research and Development Program of Jiangxi Province [20192BBGL70053]
- General Project of Jiangxi Natural Science Foundation [20202BAB206066]
- Science and Technology Program of Health Commission of Jiangxi Province [202130977]
A label-free, colorimetric, and fluorescence dual-channel sensing platform was developed for the detection of zearalenone in corn, allowing for quantitative detection as low as 0.89 µg kg-1 and visual detection as low as 2.5 µg kg-1. The formation of plasmonic gold nanoparticles aggregates based on charge neutralization by SYBR Green I eliminates instability in traditional gold nanoparticles colorimetric measurements, paving the way for accurate and label-free bioanalysis.
Mycotoxin contamination of corn has been considered a serious problem because it can accumulate in different organs or tissues via ingestion or skin contact and cause several health problems in humans. We have constructed a label-free, colorimetric, and fluorescence dual-channel sensing platform for the detection of zearalenone. Here, we demonstrate that plasmonic gold nanoparticles aggregates could be rapidly formed on the basis of charge neutralization by positively charged SYBR Green I. The sensing platform allowed quantitative detection as low as 0.89 mu g kg-1 and visual detection as low as 2.5 mu g kg-1. The charge neutralization strategy eliminates a major source of instability in conventional gold nanoparticles colorimetric measurements and paves the way for accurate, label-free bioanalysis.
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