Development of a label-free plasmonic gold nanoparticles aggregates sensor on the basis of charge neutralization for the detection of zearalenone

Mycotoxin contamination of corn has been considered a serious problem because it can accumulate in different organs or tissues via ingestion or skin contact and cause several health problems in humans. We have constructed a label-free, colorimetric, and fluorescence dual-channel sensing platform for the detection of zearalenone. Here, we demonstrate that plasmonic gold nanoparticles aggregates could be rapidly formed on the basis of charge neutralization by positively charged SYBR Green I. The sensing platform allowed quantitative detection as low as 0.89 mu g kg-1 and visual detection as low as 2.5 mu g kg-1. The charge neutralization strategy eliminates a major source of instability in conventional gold nanoparticles colorimetric measurements and paves the way for accurate, label-free bioanalysis.

Automated summary

A label-free, colorimetric, and fluorescence dual-channel sensing platform was developed for the detection of zearalenone in corn, allowing for quantitative detection as low as 0.89 µg kg-1 and visual detection as low as 2.5 µg kg-1. The formation of plasmonic gold nanoparticles aggregates based on charge neutralization by SYBR Green I eliminates instability in traditional gold nanoparticles colorimetric measurements, paving the way for accurate and label-free bioanalysis.