期刊
FOOD CHEMISTRY
卷 366, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.130527
关键词
Quantum dot nanobeads; Mycotoxins; Fluorescence-linked immunosorbent assay; Simultaneous detection; Feedstuffs
资金
- National Key Research and Devel-opment Program of China [2017YFC1600300]
A novel QBs-FLISA method was developed for simultaneous detection of AFB1 and ZEN in feedstuffs, with significantly improved detection sensitivity. Results showed good agreement between QBs-FLISA and LC-MS/MS analysis. Low detection limits and high recovery rates were achieved.
A novel dual quantum dot nanobeads-based fluorescence-linked immunosorbent assay (QBs-FLISA) was successfully developed for simultaneously detecting aflatoxin B1 (AFB1) and zearalenone (ZEN) in feedstuffs. Dual CdSe/ZnS quantum dot nanobeads with different diameters that emit red and green fluorescence were conjugated with anti-AFB1 and anti-ZEN monoclonal antibodies to prepare fluorescent probes, which greatly enhance analytical performance. Under the optimal conditions, the limits of detection for AFB1 and ZEN were 9.3 and 102.1 pg mL-1, respectively. The recoveries ranged from 82.50% to 116.21% with relative standard deviation less than 11.3%. Compared with traditional enzyme-linked immunosorbent assay, detection sensitivities of AFB1 and ZEN using QBs-FLISA were increased 20 and 5 folds, respectively. In addition, results of feedstuff samples analyzed by QBs-FLISA and liquid chromatography tandem mass spectrometry showed a good agreement (R2 = 0.99).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据