Biosynthesis, structure and functionality of starch granules in maize inbred lines with different kernel dehydration rate

In this study, we report important relationships between kernel starch and kernel dehydration rate for eight maize inbred lines with different dehydration characteristics. High-throughput RNA sequencing data of starch biosynthesis-related genes showed that kernel moisture content and dehydration rate were both associated with differential expression of most starch biosynthetic genes. Especially, kernel moisture content was positively correlated with the increased expression of SBEI and SBEIIb, thereby potentially inducing biosynthesis of amylose with low molecular weight and amylopectin with low content of amylopectin chains with degree of polymerization (DP) 6-12 in inbred lines with fast kernel dehydration rate. We found a negative correlation between short amylopectin chains (DP 6-12) and the starch retrogradation rate. Hence, a low amount of amylopectin chains with DP 6-12 in the inbred lines with fast kernel dehydration rate was a plausible reason for their high short- and long-term retrogradation.

Automated summary

The study highlights the relationship between kernel moisture content and dehydration rate of maize inbred lines, as well as the differential expression of starch biosynthetic genes. Specifically, the increased expression of certain genes in inbred lines with fast dehydration rates may lead to the synthesis of amylose with low molecular weight and amylopectin with low content of amylopectin chains, contributing to their high retrogradation rates.