Metabolomics workflow for quality control of differently-processed pre-cooked chicken fillets

The contents and profiles of small molecules in a food can provide information about quality-related properties. Processing methods and deterioration during storage, e.g. from bacterial proliferation and degradation, might also lead to changes in the metabolome, which can be determined by mass spectrometry-based metabolomics. By measuring as many metabolites as possible in differently treated pre-cooked chicken fillets in an untargeted approach, we studied individual and combined effects of vacuum packaging (VP), soluble gas stabilisation (SGS), high pressure processing (HPP), and microwave volumetric heating (MW) on the quality and shelf-life of the finished product. The extensive dataset was processed using an optimised workflow of consecutive software tools with stringent statistical analysis to prevent over-interpretation, which is an inherent risk of metabolomics data. Our results showed the predominant influence of VP on storage quality since SGS, HPP, and MW did not have the potential to extent shelf-life.

Automated summary

The contents and profiles of small molecules in food can provide quality-related information. Changes in processing methods and storage conditions can affect the metabolome, and by utilizing mass spectrometry-based metabolomics, these changes can be determined. The study showed that vacuum packaging had the most significant impact on storage quality, while other methods did not have the potential to extend shelf life.