Surface molecularly imprinted polymers fabricated by differential UV-vis spectra and reverse prediction method for the enrichment and determination of sterigmatocystin

A novel magnetic molecularly imprinted polymers (MMIPs) with a new functional monomer Triallyl isocyanurate was synthesized successfully to enrich and detect sterigmatocystin (STG) in wheat samples. The differential UV-vis spectra and the reverse prediction method were selected to achieve the optimal synthesis conditions of the MMIPs, which were characterized well. The adsorption experiment showed that MMIPs have high selectivity and sensitivity. A magnetic solid phase extraction combined with high performance liquid chromatography (MSPE-HPLC) method based on the MMIPs was successfully established with the optimal extraction condition. The linear range and RSD were 1.8-25 ng.g(-1) and 2.6-4.1%, respectively. The recovery of this method was 87.6-96.9% and the limit of detection (LOD) was 0.63 ng.g(-1). The excellent sensitivity and selectivity of this method were confirmed by experiment of the extraction and detection of STG in wheat extracts. This work extends the use of molecular imprinting in mycotoxins applications.

Automated summary

A novel magnetic molecularly imprinted polymers (MMIPs) using a new functional monomer was successfully synthesized for enrichment and detection of sterigmatocystin (STG) in wheat samples. The method showed high selectivity and sensitivity, with optimal extraction conditions and a recovery rate of 87.6-96.9%. The study extends the use of molecular imprinting in mycotoxins applications.