期刊
FOOD CHEMISTRY
卷 363, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.130250
关键词
Label-free quantification; LC-MS; MS; Quinoa; Seed; Shotgun proteomics
资金
- Spanish Ministry of Economy and Competitiveness [RTI2018-097411-B-I00]
- Cathedra UB Rector Francisco Buscarons Ubeda (Forensic Chemistry and Chemical Engineering)
- National Agricultural Innovation Program
- Ministry of Education from Peru
This study analyzed the proteins from different varieties of quinoa seeds from Peru and Bolivia using advanced LC-MS/MS technology, identifying protein similarities and differences in the proteome. The comprehensive experimental quinoa seed proteome map presented in this study serves as a valuable starting point for further characterization and nutritional studies of quinoa and quinoa-containing food products.
Quinoa seed proteins are of prime importance in human nutrition and in plant breeding for cultivar identification and improvement. In this study, proteins from seeds of black, red, white quinoa from Peru and white quinoa from Bolivia (also known as royal) were extracted, digested and analyzed by nano-liquid chromatography coupled to Orbitrap tandem mass spectrometry (LC-MS/MS). The raw mass spectra data were processed for identification and label-free quantification (LFQ) using MaxQuant/Andromeda against a specific quinoa database from The National Center for Biotechnology Information (NCBI). In total, 1,211 quinoa proteins (85 were uncharacterized) were identified. Inspection and visualization using Venn diagrams, heat maps and Gene Ontology (GO) graphs revealed proteome similarities and differences between the four varieties. The presented data provides the most comprehensive experimental quinoa seed proteome map existing to date in the literature, as a starting point for more specific characterization and nutritional studies of quinoa and quinoa-containing foodstuff.
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