期刊
FOOD CHEMISTRY
卷 384, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.132532
关键词
Human milk N -glycome; Probiotic function; Bifidobacterium longum subsp; infantis; Action mechanism; Principal functional components
资金
- National Natural Science Foundation of China (NSFC) [31871754, 31871793]
- National Key Research and Development Program of the 13th (Research on the creation and common key technologies of special dairy products based on milk components) [2017YFD0400604]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
This study demonstrates that intact human milk N-glycome is transferred into bacterial cells and decomposed intracellularly. The principal components responsible for the probiotic function of human milk N-glycome towards B. infantis ATCC 15697 are galactose-containing glycans.
Human milk N-glycome promotes the growth of Bifidobacterium longum subsp. infantis ATCC 15697. However, the action mode of, and the major functional components for, the bifidogenic function of human milk N-glycome remain unclear. In this study, we demonstrated that milk N-glycome was transferred in an intact form from culture into the bacterial cell and then decomposed intracellularly, evidenced by the following facts: (1) No UHPLC peak shift of N-glycome recovered from culture was observed. (2) No milk N-glycan specific monosugar was detected in culture supernatant. (3) High intracellular exoglycosidase activities were detected. (4) Fluorescently labeled N-glycans were found to be located intracellularly using Laser Scanning Confocal Microscopy (LSCM). Regarding the principal components identification, a novel sequential deglycosylation-based strategy was established. Degalactosylation, defucosylation-desialylation, and defucosylation-desialylationdegalactosylation treatments of human milk N-glycome showed that galactose-containing glycans were the principal components for the probiotic function of human milk N-glycome towards B. infantis ATCC 15697.
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