期刊
FOOD CHEMISTRY
卷 360, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.130008
关键词
Pesticides; Wine; QuEChERS; spectrometry; Analytical methodology; Uncertainty; Liquid chromatography-LTQ Orbitrap mass
资金
- project FoodOmicsGR Comprehensive Characterization of Foods - Operational Programme Competitiveness, Entrepreneurship and Innovation (NSRF 2014-2020) [MIS 5029057]
- European Union (European Regional Development Fund)
A sensitive and rapid method for the determination of multiclass pesticides in white and red wines was developed using QuEChERS extraction procedure and UHPLC-Orbitrap-MS. The method was optimized to improve recoveries and reduce co-extractives, and successfully applied to real samples, revealing relatively low levels of two selected fungicides.
In this research, a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction procedure and UltraHigh Performance Liquid Chromatography-Orbitrap-Mass Spectrometry (UHPLC-Orbitrap-MS), were combined to obtain a sensitive and rapid method for the determination of multiclass pesticides in white and red wines. The optimization strategy involved the selection of buffering conditions, by applying different QuEChERS procedures and sorbents for the cleanup step in order to achieve acceptably high recoveries and low co-extractives in the final extracts. Identification was based on both accurate mass and retention time, while further confirmation was achieved by MS fragmentation. The method was evaluated in terms of linearity, recovery, precision, limit of detection (LOD) and quantification (LOQ), matrix effects (ME) and expanded uncertainty. The validated method was successfully applied to real samples (home-made and commercial) revealing the presence of two selected fungicides, in relatively low levels compared to the MRLs defined by the EU for vinification grapes.
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