Integrating proteomics and metabolomics approaches to elucidate the ripening process in white Psidium guajava

Psidium guajava (guava) exhibits a high content of biomolecules with nutraceutical properties. However, the biochemistry and molecular foundation of guava ripening is unknown. We performed comparative proteomics and metabolomics studies in different fruit tissues at two ripening stages to understand this process in white guava. Our results, suggest the positive contribution of ethylene and abscisic acid (ABA) signaling to the regu-lation of biochemical changes during guava ripening. We characterized the modulation of several metabolic pathways, including those of sugar and chlorophyll metabolism, abiotic and biotic stress responses, and biosynthesis of carotenoids and secondary metabolites, among others. In addition to ethylene and ABA, we also found a differential accumulation of other growth regulators such as brassinosteroids, cytokinin, methyl-jasmonate, gibberellins and proteins, and discuss their possible implications in the intricate biochemical network associated with guava ripening process. This integrative approach represents a global overview of the metabolic pathway dynamics during guava ripening.

Automated summary

The study identified the positive roles of ethylene and abscisic acid (ABA) signaling in regulating biochemical changes during guava ripening, and characterized the modulation of various metabolic pathways. It also highlighted the differential accumulation of growth regulators and discussed their potential implications in guava ripening. This integrative approach provided a global overview of metabolic pathway dynamics during guava ripening.