Molecular-structure evolution during in vitro fermentation of granular high-amylose wheat starch is different to in vitro digestion

This study investigates the evolution of the distributions of whole molecular size and of chain length of granular wheat starches (37 similar to 93% amylose content), subjected to in vitro fermentation with a porcine faecal inoculum or digestion with pancreatic enzymes. The results showed that the molecular structures of high-amylose starch (HAS) unfermented residues largely remained unchanged during the fermentation process, while wild-type starch (37% amylose content) showed a preferential degradation of the amylopectin fraction. In contrast, under simulated digestion conditions, the undigested residues of HAS showed structural changes, including a decrease in amylose content, a shift of amylose peak position towards lower degrees of polymerisation, and an enzyme-resistant fraction. These changes of starch structure are likely to be dependent on the different starch-degrading enzyme activities present in pancreatic vs. microbial systems. Molecular changes in response to fermentation metabolism revealed by size-exclusion chromatography can help understand the microbial utilization of resistant starch.

Automated summary

This study investigated the evolution of molecular size and chain length distributions of granular wheat starches under in vitro fermentation and digestion conditions, revealing distinct structural changes between high-amylose starch (HAS) and wild-type starch. These changes were found to be dependent on the different starch-degrading enzyme activities present in pancreatic vs. microbial systems, providing valuable insights into microbial utilization of resistant starch.