4.7 Article

A fast HRMA tool to authenticate eight salmonid species in commercial food products

期刊

FOOD AND CHEMICAL TOXICOLOGY
卷 156, 期 -, 页码 -

出版社

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2021.112440

关键词

Atlantic salmon; Pacific salmon; Real-time PCR; DNA barcode; Fish authenticity; High resolution melting analysis

资金

  1. EU
  2. European Union's Horizon 2020 funding programme [773400]
  3. Fundacao para a Ciencia e Tecnologia the researcher contract [UID/QUI/50006/2019]
  4. FCT (Fundacao para a Ciencia e a Tecnologia)
  5. FEDER
  6. FCT/MEC - FEDER
  7. FCT

向作者/读者索取更多资源

The study utilized High-Resolution Melting Analysis (HRMA) to quickly identify eight salmonid species, finding that food processing does not affect its reliability, with experimental results showing a low substitution rate.
Atlantic and Pacific salmon are frequently consumed species with very different economic values: farmed Atlantic salmon is cheaper than wild-caught Pacific salmons. Species replacements occur with the high valued Pacific species (Oncorhynchus keta, O. gorbuscha, O. kisutch, O. nerka and O. tshawytscha) substituted by cheaper farmed Atlantic salmon (Salmo salar) and Atlantic salmon by rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta). Here we use High-Resolution Melting Analysis (HRMA) to identify eight salmonid species. We designed primers to generate short amplicons of 72 and 116 bp from the fish barcode genes CO1 and CYTB. The time of analysis was under 70 min, after DNA extraction. Food processing of Atlantic salmon (fresh, Bel-levue, gravadlax, frozen and smoked) did not impact the HRMA profiles allowing reliable identification. A blind test was conducted by three different institutes, showing correct species identifications irrespective of the laboratory conducting the analysis. Finally, a total of 82 retail samples from three European countries were analyzed and a low substitution rate of 1.2% was found. The developed tool provides a quick way to investigate salmon fraud and contributes to safeguard consumers.

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