期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 158, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2021.112671
关键词
Endocrine disruptors; Asthma; Mechanisms; Toxicogenomic data mining; Probiotic
资金
- Ministry of Education, Science and Technological Development of the Republic of Serbia [45103-9/2021-14/200161]
The study explored the mechanisms of DEHP, DBP, and BPA mixture-induced asthma, identifying apoptosis, inflammation, and oxidative stress as the main pathways. In vivo experiments showed significant changes in redox status and essential metals mainly in the MIX group, hinting at potential additive effects. In vitro experiments confirmed the probiotic's ability to bind the mixture and ameliorate its effects on rat tissues.
The aim of this study was to explore the mechanisms of bis(2- ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP) and bisphenol A (BPA) mixture-induced asthma development and test probiotic as a potential positive intervention. Comparative Toxicogenomics Database (CTD) and ToppGene Suite were used as the main tools for in silico analysis. In vivo 28-day experiment was conducted on rats - seven groups (n = 6): (1) Control: corn oil, (2) P: probiotic (8.78* 10(8) CFU/kg/day); (3) DEHP: 50 mg/kg b.w./day, (4) DBP: 50 mg/kg b.w./day, (5) BPA: 25 mg/kg b.w./day; (6) MIX: DEHP + DBP + BPA; (7) MIX + P. Lungs, thymus and kidneys were extracted and prepared for redox status and essential metals analysis. By conducting additional in vitro experiment, probiotic phthalate and BPA binding ability was explored. There were 24 DEHP, DBP and BPA asthma-related genes, indicating the three most probable mechanisms - apoptosis, inflammation and oxidative stress. In vivo experiment confirmed that significant changes in redox status/essential metal parameters were either prominent, or only present in the MIX group, indicating possible additive effects. In vitro experiment confirmed the ability of the multy-strain probiotic to bind DEHP/DBP/BPA mixture, while probiotic administration ameliorated mixture-induced changes in rat tissue.
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