Blumea laciniata protected Hep G2 cells and Caenorhabditis elegans against acrylamide-induced toxicity via insulin/IGF-1 signaling pathway

ABSTR A C T Acrylamide (AC), a proved toxin is mainly used in industrial fields and proved to possess various toxicities. In recent years, AC has been found in starch-containing foods due to Maillard reaction in a high-temperature process. Therefore, how to mitigate the toxic effect of AC is a research spot. Blumea laciniata is a widely used folk medicine in Asia and the extract from B. laciniata (EBL) exhibited a strong protection on cells against oxidative stress. In this work, we used EBL to protect Hep G2 cells and Caenorhabditis elegans against AC toxicity. As the results turned out, EBL increased cell viability under AC stress and notably reduced the cell apoptosis through decreasing the high level of ROS. Moreover, EBL extended the survival time of C. elegans, while EBL failed to prolong the survival time of mutants that were in Insulin signaling pathway. Besides, the expressions of antioxidant enzymes were activated after the worms were treated with EBL and daf-16 gene was activated. Our results indicated that EBL exhibited a protective effect against AC induced toxicity in Hep G2 cells and C. elegans via Insulin/IGF-1 signaling pathway. These outcomes may provide a promising natural drug to alleviate the toxic effect of AC.

Automated summary

In this study, the extract from Blumea laciniata (EBL) was used to protect Hep G2 cells and Caenorhabditis elegans against acrylamide (AC) toxicity. The results showed that EBL could mitigate the toxic effects of AC by reducing ROS levels, activating antioxidant enzyme expression, and enhancing cell or worm survival time. Additionally, EBL exhibited a protective effect via the Insulin/IGF-1 signaling pathway.