期刊
FOOD ANALYTICAL METHODS
卷 15, 期 4, 页码 847-855出版社
SPRINGER
DOI: 10.1007/s12161-021-02169-z
关键词
DNA motor; One-step; Self-powered; Ochratoxin A; DNAzyme
资金
- Chongqing Key Research Base of Humanities and Social Sciences Chongqing Research Center of Overall Development of Urban-Rural Teachers' Education [JDND 201707]
- Innovation Center of Lipid Resources and Children's Daily Chemicals at Chongqing University of Education [2017XJPT01]
- Chongqing University of Eduacation [CRIKT202021]
- Children's Research Institute of National Center for Schooling Development Programme [CRIKT202021]
A programmed, autonomous, and self-powered DNA motor was developed for one-step amplification detection of ochratoxin A. The motor undergoes autonomous and processive movement by circular cleaving fluorophore-labeled DNA on gold nanoparticles. This self-powered strategy shows great potential in food security supervision or biological imaging of OTA.
A programmed, autonomous, and self-powered DNA motor was developed for one-step amplification detection of ochratoxin A (OTA). The OTA can bind with the aptamer of hairpin and induce the opening of hairpin. Then, the Mg2+-specific DNAzyme can circularly cleave the fluorophore-labeled DNA on the gold nanoparticles (AuNPs), causing autonomous and processive movement of DNA motor. Finally, the cleaved fluorophore-labeled DNA segments can leave from the AuNP surface, causing significant recovery of fluorescent signal. A good linear range was obtained from 0.01 to 5 nM (R-2 = 0.992) with a detection limit of 2 pM. Importantly, this strategy is self-powered and only one-step operation is needed for the whole process. It shows great potential in food security supervision or biological imaging of OTA.
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