Glutamate dehydrogenase enables Salmonella to survive under oxidative stress and escape from clearance in macrophages

Oxidative stress and the production of reactive oxygen species (ROS) are a biological threat to bacteria, which induce the synthesis of proteins and production of antioxidants to combat it. Herein, we report that glutamate dehydrogenase (GDH) of Salmonella can assimilate ammonium into glutamate and promote the generation of glutathione (GSH) to combat oxidative damage. Oxidation induces the transcription of gdhA, which encodes GDH, and activates the enzymatic activity of GDH. The Delta gdhA mutant Salmonella strain showed decreased levels of GSH and reduced survival in macrophages, and this growth deficiency could be partially restored by overexpression of GDH and complementation with its downstream metabolites. Therefore, GDH plays a critical role in the growth of Salmonella in oxidative environments, especially under low energy supply.

Automated summary

The study shows that the glutamate dehydrogenase (GDH) of Salmonella plays a critical role in combating oxidative damage by promoting the generation of glutathione (GSH). GDH is particularly important for the growth of Salmonella in oxidative environments under low energy supply.