期刊
FEBS LETTERS
卷 596, 期 15, 页码 1932-1943出版社
WILEY
DOI: 10.1002/1873-3468.14322
关键词
acetyl xylan esterase; carbohydrate esterase family 1; feruloyl esterase; fungi; hydroxycinnamic acid; plant biomass
资金
- Genome Canada
- Genome Quebec
- China Scholarship Council [201803250066]
In this study, ten novel fungal CE1 enzymes from different subfamilies were heterologously produced and screened for their activity towards model and complex plant biomass substrates. The study found CE1 enzymes with dual feruloyl/acetyl xylan esterase (FXE) activity, expanding the substrate specificity. These new FXEs from CE1 can efficiently release both feruloyl and acetyl residues from feruloylated xylan, making them potentially valuable components in industrial enzyme cocktails for plant biomass degradation.
Feruloyl esterases (FAEs) and acetyl xylan esterases (AXEs) are important enzymes for plant biomass degradation and are both present in Carbohydrate Esterase family 1 (CE1) of the Carbohydrate-Active enZymes database. In this study, ten novel fungal CE1 enzymes from different subfamilies were heterologously produced and screened for their activity towards model and complex plant biomass substrates. CE1_1 enzymes possess AXE activity, while CE1_5 enzymes showed FAE activity. Two enzymes from CE1_2 and one from CE1_5 possess dual feruloyl/acetyl xylan esterase (FXE) activity, showing expansion of substrate specificity. The new FXEs from CE1 can efficiently release both feruloyl and acetyl residues from feruloylated xylan, making them particularly interesting novel components of industrial enzyme cocktails for plant biomass degradation.
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