期刊
EUROPEAN JOURNAL OF PHARMACOLOGY
卷 908, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ejphar.2021.174353
关键词
Glycyrrhizic acid; Ischemia-reperfusion; Endoplasmic reticulum stress; Myocardium; Inflammation
资金
- National Natural Science Foundation of China [81860797]
- Guangxi Natural Science Foundation [2019JJA140159]
- Science and Technology Fund of Guangxi Health Commission [Z2014607]
- Guangxi Young and Middle-aged Teachers' Basic Ability Improvement Fund [2018KY0450]
This study demonstrates the cardioprotective role of glycyrrhizic acid in myocardial ischemia-reperfusion injury may involve attenuation of endoplasmic reticulum stress-induced apoptosis and inflammation, potentially providing an alternative strategy for intervention of myocardial ischemia-reperfusion injury.
The purpose of this study was to investigate the role of glycyrrhizic acid (GA) in regulating myocardial ischemiareperfusion injury (MIRI) in rats as well as the underlying mechanism. H9c2 cells were subjected to hypoxia/reoxygenation (H/R) to mimic the MIRI in vitro, while a rat model of ischemia-reperfusion (I/R) was constructed by occlusion of the left anterior descending coronary artery for 0.5 h followed by 2 h of reperfusion. While flow cytometry and TUNEL assay were performed to analyze apoptosis in cells and myocardial tissue, echocardiography, hematoxylin and eosin staining, and Masson's trichrome staining were conducted to evaluate cardiac function and pathological changes, respectively. The levels of serum CK, CK-MB, LDH, AST, TNF-alpha, and IL-6 as well as the contents of MDA and SOD in tissues were measured by ELISA, while Western blot analysis was performed to detect the expression of endoplasmic reticulum stress (ERS)-related proteins. GA treatment significantly reduced apoptosis in H9c2 cells, while it alleviated left ventricular dysfunction, fibrosis and myocardial apoptosis, down-regulated the levels of CK, CK-MB, LDH, AST, TNF-alpha, IL-6, and MDA, and upregulated SOD levels in I/R rats. Moreover, GA treatment led to a decrease in the expression of CHOP, GRP78, and p-PERK in both H/R cells and I/R rats. This study demonstrates that cardioprotective role of GA in MIRI may involve the attenuation of ERS-induced apoptosis and inflammation, potentially providing an alternative strategy for intervention of MIRI.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据