期刊
EUROPEAN JOURNAL OF NUTRITION
卷 61, 期 4, 页码 1919-1929出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00394-021-02748-0
关键词
Angiopoietin-like protein 3; Angiopoietin-like protein 4; Oral lipid tolerance test; Oral glucose tolerance test; Adipocyte; Adipose tissue; Triglyceride
资金
- Projekt DEAL
The study revealed short-term regulation of Angptl3/4 levels under metabolic stress, with high expression of Angptl4 and low expression of Angptl3 in adipose tissue, mainly in mature adipocytes. Omega-3/-6 polyunsaturated fatty acids inhibited Angptl3 expression in adipocytes, while dietary fatty acids and glucose had no effect.
Background The postprandial regulation of angiopoietin-like proteins (Angptls) and their expression in adipocytes is poorly characterized. Objective Circulating Angptl3 and 4 were analyzed in healthy individuals undergoing either an oral lipid tolerance test (OLTT; n = 98) or an oral glucose tolerance test (OGTT; n = 99). Venous blood was drawn after 0, 2, 4, and 6 h during OLTT and after 0, 1, and 2 h during OGTT. Anthropometric and laboratory parameters were assessed and concentrations of Angptls were quantified by enzyme-linked immunosorbent assay. Angptl gene expression in 3T3-L1 adipocytes and in murine adipose tissues and cellular fractions was analyzed by quantitative real-time PCR. Results Angptl3 concentrations significantly decreased while Angptl4 levels continuously increased during OLTT. Both proteins remained unaffected during OGTT. Angptl3 and Angptl4 were expressed in murine subcutaneous and visceral AT with higher mRNA levels in mature adipocytes when compared to the stroma-vascular cell fraction. Both proteins were strongly induced during 3T3-L1 adipocyte differentiation and they were unresponsive to glucose in mature fat cells. Adipocyte Angptl3 (but not Angptl4) mRNA expression was inhibited by the polyunsaturated fatty acids arachidonic acid and docosahexaenoic acid, whereas nine types of dietary fatty acids remained without any effect. Conclusions There is evidence of short-time regulation of Angptl3/4 levels upon metabolic stress. Angptl4 expression is high and Angptl3 expression is low in AT and restricted mainly to mature adipocytes without any differences concerning fat compartments. Whereas dietary fatty acids and glucose are without any effect, omega-3/-6-polyunsaturated fatty acids inhibited Anptl3 expression in adipocytes.
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