期刊
BULLETIN OF ENTOMOLOGICAL RESEARCH
卷 105, 期 2, 页码 202-209出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007485315000024
关键词
COI barcode gene; flat grain beetles; molecular diagnosis; species-specific primer
类别
资金
- International Science and Technology Cooperation Program of China [2013DFG32350]
- National Quality Supervision, Inspection and Quarantine Public Welfare Industry Research Project of China [201310075]
- Charles University in Prague [SVV 260 087/2014]
- Oklahoma Agricultural Experiment Station [OKL02695]
- [KONTAKT - LH12160]
Flat grain beetles of the genus Cryptolestes (Coleoptera: Laemophloeidae) are one of the economically most important stored-product pests which feed on many kinds of agricultural products, especially grains. Nine of more than 40 described Cryptolestes species are recognized as stored-product pests and two of the pest species have a cosmopolitan distribution. Given the rapid growth in global trade of food products, ecological barriers to the spread of pests are easily overcome. Therefore, development of reliable systems for routine quarantine inspection and early infestation detection is vital. In the present study, we established a new rapid and accurate cytochrome c oxidase subunit I-based system for molecular identification of five common stored-product Cryptolestes species, namely, Cryptolestes capensis, Cryptolestes ferrugineus, Cryptolestes pusilloides, Cryptolestes pusillus and Cryptolestes turcicus. Five species-specific primer pairs for traditional uniplex polymerase chain reaction assay are described and their specificity and sensitivity for the identification process is evaluated using larval samples of 12 different populations from three continents (Asia, Europe and North America).
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