期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 150, 期 -, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2021.109886
关键词
Metabolic engineering; Escherichia coli; Tyrosol acetate; Hydroxytyrosol acetate
资金
- National Natural Science Foundation of China [31960216]
- National Science Foundation of Jiangxi Province [220192BBG70007, 20192BCBL23012]
A new biosynthetic pathway was constructed in Escherichia coli for the production of tyrosol acetate and hydroxytyrosol acetate, starting with the production of tyrosol and extending the pathway with the overexpression of alcohol acetyltransferase and hydroxylase enzymes. This study provides a novel approach for biosynthesis of these bioactive compounds with potentially increased bioavailability for various applications in food and cosmetics industries.
Tyrosol and hydroxytyrosol derived from virgin olive oil and olives extract, have wide applications both as functional food components and as nutraceuticals. However, they have low bioavailability due to their low absorption and high metabolism in human liver and small intestine. Acetylation of tyrosol and hydroxytyrosol can effectively improve their bioavailability and thus increase their potential use in the food and cosmeceutical industries. There is no report on the bioproductin of tyrosol acetate and hydroxytyrosol acetate so far. Thus, it is of great significance to develop microbial cell factories for achieving tyrosol acetate or hydroxytyrosol acetate biosynthesis. In this study, a de novo biosynthetic pathway for the production of tyrosol acetate and hydroxytyrosol acetate was constructed in Escherichia coli. First, an engineered E. coli that allows production of tyrosol from simple carbon sources was established. Four aldehyde reductases were compared, and it was found that yeaE is the best aldehyde reductase for tyrosol accumulation. Subsequently, the pathway was extended for tyrosol acetate production by further overexpression of alcohol acetyltransferase ATF1 for the conversion of tyrosol to tyrosol acetate. Finally, the pathway was further extended for hydroxytyrosol acetate production by overexpression of 4-hydroxyphenylacetate 3-hydroxylase HpaBC.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据