期刊
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
卷 88, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.etap.2021.103748
关键词
Triclosan; Larvae; Biochemical changes; Digestive genes; Transcriptomic alterations
资金
- Council of Scientific and Industrial research (CSIR), India
- Uni-versity Grants Commission Special Assistance Programme (UGC-SAP)
The study revealed significant effects of Triclosan on biochemical parameters and gene expression in Labeo rohita larvae, with certain biomarkers like glucose, triglycerides, urea, uric acid, and alkaline phosphatase showing significant declines after exposure. Some parameters showed opposite trends during the recovery period, suggesting their importance in identifying the health status of fish under TCS stress.
In the current study, Triclosan (TCS, a commonly used antimicrobial agent) induced alterations in biochemical parameters and gene expression were recorded in the larvae of Labeo rohita after 96 h exposure and 10 days recovery period to find out health status biomarkers. 96 h exposure to 0.06, 0.067 and 0.097 mg/L TCS significantly declined the levels of glucose, triglycerides, urea and uric acid and activity of alkaline phosphatase (ALP), glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT). There was a nonsignificant decline in the levels of cholesterol and total protein but albumin and total bilirubin showed no change. After 10 days of recovery period, trend was opposite for glucose, urea and ALP only. Decline in the expression of trypsin and pancreatic amylase and elevation in creatine kinase during exposure to TCS showed a reverse trend after recovery period. However, concentration dependent elevation of chymotrypsin persisted till the end of recovery period. Principal Component Analysis (PCA) showed association of total protein, ALP, GOT, creatine kinase and pancreatic amylase with PC1 after exposure as well as recovery period. Therefore, these can be considered as important biomolecules for identification of health status of TCS stressed fish.
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