Uptake and accumulation of naphthalene, phenanthrene, and benzo(b)fluoranthene in winter wheat affected by foliar exposure at different growth stages

Foliar uptake, as an important pathway of polycyclic aromatic hydrocarbons (PAHs) accumulation in winter wheat, has a great contribution to wheat PAHs, which mainly depends on atmospheric PAHs level. An indoor simulation experiment was conducted to explore the effects of foliar exposure to PAHs at different growth stages on PAHs uptake in wheat. Three levels (0, 0.75, 4.5 mg L-1) of mixed solution of three PAHs (sigma(3)PAHs) including naphthalene (NAP), phenanthrene (PHE), and benzo(b)fluoranthene (BbF) were sprayed on leaves of two varieties (Yunong, YN; Xiaoyan, XY) of winter wheat (Triticum aestivum L.) during the booting, heading, pre-filling, and post-filling stage. The results showed that the sprayed PAHs exhibited little effects on the growth of the two varieties except the stem biomass of YN was significantly (p < 0.05) reduced when high concentration of PAHs was applied at the post-filling stage. PAHs concentration in winter wheat grain was highest under foliar exposure at the pre-filling stage, while the lowest was found under foliar exposure at the post-filling stage. Transfer factor of PAHs from stem to root (TFroot/stem) of three PAHs when foliar exposure to PAHs at the booting and heading stage was significantly (p < 0.001) higher than that at the pre-filling and post-filling stage, while TFgrain/glume of three PAHs when foliar exposure to PAHs at pre-filling stage was significantly (p < 0.01) higher than that at the other three stages. These results indicated that foliar exposure to PAHs during the vegetative growth stage was transferred and distributed to the root, while PAHs are mainly transferred and accumulated to the grain during the grain filling stage. Additionally, the higher lipophilic PAHs showed a lower ability to transfer from the glume to grain, and larger flag leaf area had the potential to promote the enrichment of PAHs in grain. This study indicated that the health risk of PAHs in winter wheat could be effectively reduced by controlling atmospheric PAHs level during pre-filling stage.

Automated summary

Foliar uptake is an important pathway for polycyclic aromatic hydrocarbons (PAHs) accumulation in winter wheat. This study found that foliar exposure to PAHs at different growth stages affects PAHs uptake in wheat and that PAHs are mainly transferred to the root and grain during the grain filling stage.