The distribution and retained amount of benzo[a]pyrene at the micro-zones of mangrove leaf cuticles: Results from a novel analytical method

Plant leaf cuticles play a critical role in the accumulation and transport of atmospheric polycyclic aromatic hydrocarbons (PAHs). The relationship between the distribution and retained amount of PAHs on the leaf cuticles and the leaves micro-zone structures is still unclear. In this study, a confocal microscopic fluorescence spectral analysis (CMFSA) system with a spatial resolution of 200 nm was established as a direct and noninvasive means to determine the microscopic distribution and quantify the retained amount of benzo[a]pyrene (B[a]P) at Aegiceras corniculatum (Ac), Kandelia obovata (Ko) and Avicennia marina (Am) leaf cuticle micro-zones (0.096 mm(2)). The linear ranges for the established method were 10-1900 ng spot(-1) for Ac, 15-1700 ng spot(-1) for Ko and 30-1800 ng spot(-1) for Am, and the detection limits were 0.06 ng spot(-1) for Ac, 0.06 ng spot(-1) for Ko and 0.07 ng spot(-1) for Am. Notably, B[a]P formed clusters and unevenly distributed at the leaf cuticles. On the adaxial cuticles, B[a]P was mainly accumulated unevenly along the epidermis cell wall, and it was also distinctively distributed in the secretory cells around salt glands for Ac and Am. On the abaxial leaf cuticles, B[a]P was concentrated in the salt glands and stomata apart from being unevenly distributed in the epidermis cell wall. Moreover, the amount of B[a]P retained presented a negative correlation with the polarity of leaf cuticles, which resulted in the amount of B[a]P retained on the adaxial leaf cuticles being significantly higher than that on abaxial leaf cuticles. Our results provide a potential in situ method for investigating the distribution and retained amount of PAHs at plant leaf surface micro-zones, which would contribute to further studying and understanding the mechanism of migration and transformation of PAHs by plant leaves from a microscopic perspective.

Automated summary

The study reveals that the distribution and retained amount of PAHs on plant leaf surface micro-zones are closely related to the microstructure of the leaf cuticle, with B[a]P showing uneven distribution on the leaf cuticles. Additionally, there is a negative correlation between the polarity of leaf cuticles and the amount of B[a]P retained, resulting in a significantly higher retention of B[a]P on the adaxial leaf cuticles compared to the abaxial leaf cuticles.