Roles of exogenous plant growth regulators on phytoextraction of Cd/Pb/Zn by Sedum alfredii Hance in contaminated soils

Plant growth regulators (PGRs) assisted phytoextraction was investigated as a viable phytoremediation technology to increase the phytoextraction efficiency in contaminated soils. This study aimed to evaluate the cadimum (Cd)/lead (Pb)/zinc (Zn) phytoextraction efficiency by a hyperaccumulator Sedum alfredii Hance (S. alfredii) treated with 9 PGRs, including indole-3-acetic acid (IAA), gibberellin (GA(3)), cytokinin (CKs), abscisic acid (ABA), ethylene (ETH), brassinosteroid (BR), salicylic acid (SA), strigolactones (SL) and jasmonic acid (JA), in slightly or heavily contaminated (SC and HC, respectively) soil. Results demonstrated that PGRs were able to improve S. alfredii biomass, the most significant increases were observed in GA(3) and SL for HC soil, while for SC soil, IAA and BR exhibited positive effects. The levels of Cd, Pb and Zn in the shoots of S. alfredii treated with ABA and SL were noticeably greater than in the CK treatment in HC soil, while the uptake of metals were increased by IAA and CKs in SC soil. Combined with the results of biomass and metal contents in shoots, we found that ABA showed the highest Cd removal efficiency and the maximum Pb and Zn removal efficiency was observed with GA(3), which was 62.99%, 269.23%, and 41.18%, respectively higher than the control in HC soil. Meanwhile, compared to control, the maximum removal efficiency of Cd by IAA treatment (52.80%), Pb by JA treatment (165.1%), and Zn by BR treatment (44.97%) in the SC soil. Overall, our results suggested that these PGRs, especially, ABA, SL, IAA, BR and GA(3) had great potential in improving phytoremediation efficiency of S. alfredii grown in contaminated soils.

Automated summary

This study investigated the use of plant growth regulators (PGRs) to enhance the phytoextraction efficiency of hyperaccumulator Sedum alfredii Hance in contaminated soils. Various PGRs, including ABA, SL, IAA, BR, and GA(3), were found to significantly improve biomass and metal uptake of S. alfredii. ABA showed the highest Cd removal efficiency, while GA(3) exhibited the maximum Pb and Zn removal efficiency. Overall, these PGRs have great potential in improving phytoremediation efficiency in contaminated soils.