Diversity and distribution of antibiotic resistance genes associated with road sediments transported in urban stormwater runoff

Recently, increasing attention has been paid to antibiotic resistance genes (ARGs) in urban stormwater runoff. However, there were little data on the diversity and distribution of ARGs associated with road sediments transported in runoff. The investigation of ARGs diversity showed that sulfonamide resistance genes (sul2 and sul3) occupied 61.7%-82.3% of total ARGs in runoff. The analysis of ARGs distribution in particulate matter (PM) implied that both tetQ and trbC existed mainly in PM with size of 150-300 mu m, but other ARGs and mobile genetic elements (MGEs) were dominant in PM with size <75 mu m. The discussion of potential hosts indicated that target genes (ermF, blaOXA1/blaOXA30, ermC, qnrA, sul2, tnpA-01, intI2, tetW, intI1, sul3, trbC) had the strongest subordinate relationship with Proteobacteria at phylum level and Enterobacter at genus level. The effect evaluation of ARGs distribution suggested that 13 kinds of ARGs were positively correlated with Pr/PS and Zeta potential, resulting in the more ARGs in PM with smaller size (<75 mu m).

Automated summary

Recent studies have focused on antibiotic resistance genes (ARGs) in urban stormwater runoff, with sulfonamide resistance genes found to make up a significant portion of total ARGs. Research indicated that certain ARGs were mainly distributed in larger particulate matter, while others were dominant in smaller particulate matter.