期刊
EMBO REPORTS
卷 22, 期 12, 页码 -出版社
WILEY
DOI: 10.15252/embr.202051503
关键词
cytokinesis; Ect2; Midbody; Plk1; beta-Catenin p-S60
资金
- National Research Foundation of Korea (NRF) - Korean government (MSIT) [NRF-2021R1A2C1004854, NRF-2021R1A2C3004965]
- R&D Convergence Program of National Research Council of Science & Technology (NST) of Republic of Korea [CAP-16-03-KRIBB]
- KRIBB Research Initiative Program
The study identifies a novel p-S60 epitope on beta-catenin generated by Plk1 kinase activity, which is crucial for the completion of cytokinesis. Depletion of beta-catenin leads to cytokinesis-defective phenotypes and apoptotic cell death, highlighting the importance of beta-catenin phosphorylation in cell division.
beta-Catenin is a multifunctional protein and participates in numerous processes required for embryonic development, cell proliferation, and homeostasis through various molecular interactions and signaling pathways. To date, however, there is no direct evidence that beta-catenin contributes to cytokinesis. Here, we identify a novel p-S60 epitope on beta-catenin generated by Plk1 kinase activity, which can be found at the actomyosin contractile ring of early telophase cells and at the midbody of late telophase cells. Depletion of beta-catenin leads to cytokinesis-defective phenotypes, which eventually result in apoptotic cell death. In addition, phosphorylation of beta-catenin Ser60 by Plk1 is essential for the recruitment of Ect2 to the midbody, activation of RhoA, and interaction between beta-catenin, Plk1, and Ect2. Time-lapse image analysis confirmed the importance of beta-catenin phospho-Ser60 in furrow ingression and the completion of cytokinesis. Taken together, we propose that phosphorylation of beta-catenin Ser60 by Plk1 in cooperation with Ect2 is essential for the completion of cytokinesis. These findings may provide fundamental knowledge for the research of cytokinesis failure-derived human diseases.
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