期刊
EMBO REPORTS
卷 22, 期 11, 页码 -出版社
WILEY
DOI: 10.15252/embr.202152389
关键词
cardiac neural crest cell; embryonic development; mural cell; neonatal development; single-cell RNA-seq
资金
- National Natural Science Foundation of China [81900282, 82070326]
- CAMS Initiative for Innovative Medicine [2016-I2M-1-016]
- Post-doctoral International Exchange Project [2018-BSH04]
- China Postdoctoral Science Foundation [2021TQ0049]
- Foundation for Fuwai Hospital Youth Scholars [2019-F08]
The study conducted single-cell transcriptomic sequencing of CNCC-derived cells in mouse hearts, revealing the presence of mural cells and the transition key regulators from pericytes to mVSMCs. Data suggests many CNCC derivatives had already committed or differentiated to a specific lineage when migrating into the heart.
The migratory cardiac neural crest cells (CNCCs) contribute greatly to cardiovascular development. A thorough understanding of the cell lineages, developmental chronology, and transcriptomic states of CNCC derivatives during normal development is essential for deciphering the pathogenesis of CNCC-associated congenital anomalies. Here, we perform single-cell transcriptomic sequencing of 34,131 CNCC-derived cells in mouse hearts covering eight developmental stages between E10.5 and P7. We report the presence of CNCC-derived mural cells that comprise pericytes and microvascular smooth muscle cells (mVSMCs). Furthermore, we identify the transition from the CNCC-derived pericytes to mVSMCs and the key regulators over the transition. In addition, our data support that many CNCC derivatives had already committed or differentiated to a specific lineage when migrating into the heart. We explore the spatial distribution of some critical CNCC-derived subpopulations with single-molecule fluorescence in situ hybridization. Finally, we computationally reconstruct the differentiation path and regulatory dynamics of CNCC derivatives. Our study provides novel insights into the cell lineages, developmental chronology, and regulatory dynamics of CNCC derivatives during development.
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