期刊
EMBO MOLECULAR MEDICINE
卷 14, 期 2, 页码 -出版社
WILEY
DOI: 10.15252/emmm.201911814
关键词
DDR; extracellular matrix; melanoma; NF-kappa B2; therapeutic resistance
资金
- Institut National de la Sante et de la Recherche Medicale (Inserm)
- Ligue Contre le Cancer
- Institut National du Cancer [INCA_12673]
- ITMO Cancer Aviesan (Alliance Nationale pour les Sciences de la Vie et de la Sante, National Alliance for Life Science and Health) within the Cancer Plan
- French Government (National Research Agency, ANR) through the Investments for the Future LABEX SIGNALIFE [ANR-11-LABX-0028-01]
- Investments for the Future [ANR-10-INBS-04]
- Conseil general 06
- Canceropole Provence Alpes Cote d'Azur
- Region Provence Alpes Cote d'Azur
The study reveals that physical and structural signals from fibroblast-derived ECM can cause the antiproliferative responses to BRAF/MEK inhibitors to fail in melanoma. Drug-induced linear clustering of DDR1 and DDR2 mediates ECM-mediated drug resistance. Targeting DDR1 and DDR2 can overcome resistance to BRAF-targeted therapy mediated by ECM.
Resistance to BRAF/MEK inhibitor therapy in BRAF(V600)-mutated advanced melanoma remains a major obstacle that limits patient benefit. Microenvironment components including the extracellular matrix (ECM) can support tumor cell adaptation and tolerance to targeted therapy; however, the underlying mechanisms remain poorly understood. Here, we investigated the process of matrix-mediated drug resistance (MMDR) in response to BRAF(V600) pathway inhibition in melanoma. We demonstrate that physical and structural cues from fibroblast-derived ECM abrogate antiproliferative responses to BRAF/MEK inhibition. MMDR is mediated by drug-induced linear clustering of phosphorylated DDR1 and DDR2, two tyrosine kinase collagen receptors. Depletion and pharmacological targeting of DDR1 and DDR2 overcome ECM-mediated resistance to BRAF-targeted therapy. In xenografts, targeting DDR with imatinib enhances BRAF inhibitor efficacy, counteracts drug-induced collagen remodeling, and delays tumor relapse. Mechanistically, DDR-dependent MMDR fosters a targetable pro-survival NIK/IKK alpha/NF-kappa B2 pathway. These findings reveal a novel role for a collagen-rich matrix and DDR in tumor cell adaptation and resistance. They also provide important insights into environment-mediated drug resistance and a preclinical rationale for targeting DDR signaling in combination with targeted therapy in melanoma.
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