期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 230, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2021.113113
关键词
Housefly; Heavy metal; Transport; Detoxification; Luminal pH; Metal binding peptide
资金
- Natural Science Foundation of China [31872306]
The study found that regulating the expression levels of CPA1, GST2, and CYTb-c1 genes through RNA interference can reduce the accumulation of Pb in the larvae of houseflies. These proteins play crucial roles in Pb transport and detoxification in M. domestica larvae.
Lead (Pb) is a typical toxic contamination source all over the world. In this research, larvae of the housefly (Musca domestica) were fed a Pb-contaminated diet at different Pb doses of 0, 20 and 5000 mg/kg. RNA sequencing was used to identify genes that were differentially expressed in relation to lead transport or detoxification. RNA interference (RNAi) was carried on 12 candidate genes. The results showed that three luminal pH regions of mid-gut were at pH values of 6.33, 3.10, and 7.80. With increasing Pb concentration, the pH of the middle mid-gut decreased by one unit. The expression levels of carboxypeptidase A (CPA1), glutathione Stransferase (GST), and cytochrome b (Cyt b) were linked to Pb treatments, particularly high Pb concentration of 5000 mg/kg. RNAi-mediated down expression of CPA1, GST2, and CYTb-c1 resulted in low Pb accumulation in the larvae of 5000 mg/kg Pb group. These proteins played key roles in Pb transport and detoxification in M. domestica larvae.
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