期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 230, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2021.113105
关键词
Simazine; Oocyte maturation; Spindle; Actin; Mitochondria; Apoptosis
资金
- National Natural Science Foundation of China [32000581]
- Fundamental Research Funds for the Provincial Universities of Zhejiang [2020YQ007]
- Zhejiang A&F University Talent Initiative Project [2020FR033, 2019FR0022]
Exposure to Simazine reduces oocyte maturation competence and embryonic developmental capacity, disrupts microtubule stability and actin polymerization, leading to mitochondrial dysfunction and apoptosis.
Simazine is a triazine pesticides that typically detected in ground water and soil, and can reportedly affect reproductive health in humans and animals. However, the effect of simazine on female germ cell development remains unclear. In the present study, we observed that simazine exposure decreased oocyte maturation competence and embryonic developmental capacity. Importantly, simazine exposure disrupted microtubule stability and actin polymerization, resulting in failure of spindle assembly and migration. In addition, simazine exposure impaired mitochondrial function and cytosolic Ca2+ homeostasis in both oocyte and 2-cell embryos, thus increasing the levels of reactive oxygen species (ROS). Moreover, simazine exposure induced DNA damage and early apoptosis during oocyte maturation. Collectively, our results demonstrate that simazine exposureinduced mitochondrial dysfunction and apoptosis are major causes of poor oocytes quality.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据